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biotinylated erythrina cristagalli lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated erythrina cristagalli lectin
    Biotinylated Erythrina Cristagalli Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-1145-5/Biotinylated+Erythrina+Cristagalli+Lectin+(ECL%2C+ECA)/10__1038_slash_s41598___026___71900___9-103-33-39
    Average 95 stars, based on 112 article reviews
    biotinylated erythrina cristagalli lectin - by Bioz Stars, 2026-10
    95/100 stars

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    Incubation:

    Article Title: Antigen flexibility supports the avidity of hemagglutinin-specific antibodies at low antigen densities
    Article Snippet: Remaining BSA-biotin was washed with an excess of PBS, then incubated with 25 μg/mL streptavidin (Invitrogen) in PBS for 1 hour. .. Flow chambers were washed again with excess PBS and subsequently incubated with 25 μg/mL biotinylated Erythrina cristagalli lectin (ECL; Vector Laboratories) for 2 hours. ..

    Article Title: Antigen flexibility supports the avidity of hemagglutinin-specific antibodies at low antigen densities.
    Article Snippet: Remaining BSA-biotin was PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013862 February 5, 2026 11 / 17 washed with an excess of PBS, then incubated with 25 μg/mL streptavidin (Invitrogen) in PBS for 1 hour. .. Flow chambers were washed again with excess PBS and subsequently incubated with 25 μg/mL biotinylated Erythrina cristagalli lectin (ECL; Vector Laboratories) for 2 hours. ..

    Article Title: CHO cell production of a single, enveloped VLP vaccine co-displaying SARS-CoV-2, influenza and RSV antigens
    Article Snippet: .. For lectin blotting, membranes were then physically sectioned prior to incubation, and separate sections were probed with biotinylated Maackia amurensis lectin II (MAL II [1:500; Vector Laboratories, B-1265]) to detect α2,3-linked sialoglycoproteins or biotinylated Erythrina cristagalli lectin (ECL [1:500; Vector Laboratories, B-1145-5]) to detect terminal galactose residues. .. Both were followed by incubation with HRP-conjugated streptavidin (1:2000; BD Pharmingen, 554066).

    Article Title: Gne deletion in adult mice can cause thrombocytopenia, anemia, myopathy, bleeding, and death.
    Article Snippet: .. Biotinylated peanut agglutinin (PNA, B-1075-5), erythrina cristagalli lectin (ECA, B-1145-5), sambucus nigra lectin (SNA, B-1305-2), and maackia amurensis lectin II (MAA, B-1265-1) (all from Vector Laboratories; Plain City, OH) were added to the appropriate muscle sections along with rat monoclonal anti-laminin 2, α2 chain (L0663; Sigma Aldrich; St Louis, MO) and incubated overnight at 4 °C. .. Cy3-conjugated streptavidin (Jackson ImmunoResearch; West Grove, PA) and AlexaFluor 647-conjugated anti-rat IgG (Invitrogen; Waltham, MA) were added for an hour at room temperature, then slides were coverslipped using ProLong Gold antifade mountant with DAPI (ThermoFisher Scientific; Waltham, MA) and protected from light until imaging.

    other:

    Article Title: Single-cell mapping of the glycocalyx in the adult human and mouse ocular surface
    Article Snippet: DNA oligonucleotides for lectin conjugation were 21 synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Article Title: Pharmacologic glycoengineering of Fcγ receptor IIIa enhances force-resistant IgG-FcγR interactions and anti-tumor antibody efficacy.
    Article Snippet: Biological samples Healthy human peripheral blood mononuclear cells Stanford Blood Center N/A Chemicals, peptides, and recombinant proteins Fucosyltransferase inhibitor Millipore Sigma Cat#344827-10MG Protein G Sepharose Cytiva Cat# GE17-0618-02 pH 2.7 buffer Teknova Cat#P3727 Tris-HCl, pH9 Teknova Cat#T1090 Swainsonine Cayman Chemical Cat#16860 Ni Sepharose beads Milipore Sigma Cat#GE1-5268-01 Ultra centrifugal filters Milipore Sigma Cat#UFC903008/UFC901008 IL-2 Miltenyi Biotec Cat#130-097-742 CFSE dye Thermo Fisher Scientific Cat#C34554 LIVE/DEAD Fixable Near-IR dye Thermo Fisher Scientific Cat#L34975 GolgiSTOP BD Biosciences Cat#554724 LIVE/DEAD Fixable Violet dye Thermo Fisher Scientific Cat#L34955 ACK Lysing Buffer Thermo Fisher Scientific Cat#A1049201 DAPI Invitrogen Cat#D21490 Recombinant His-tagged mouse mannanbinding lectin (MBL-2) SinoBiological Cat#50063-M07H Recombinant His-tagged mouse macrophage mannose receptor (MMR/CD206 R&D Systems Cat#2535-MM-050/CF LIVE/DEAD Fixable Blue Dead Cell Stain Kit Invitrogen Cat#L23105 LIVE/DEAD Fixable Aqua Dead Cell Stain Invitrogen Cat#L34957 Hippeastrum hybrid (Amaryllis) (HHA) lectin Vector Laboratories Cat# L-1380 Pacific Orange labeling dye Thermo Fisher Scientific Cat#P30014 Biotin protein labeling kit Roche Cat#11418165001 Erythrina Cristagalli lectin (ECL, ECA), biotinylated Vector Laboratories Cat# B-1145 Streptavidin-APC BioLegend Cat#405207 FITC conjugation kit Abcam Cat#ab188285 EnVision+ System- HRP Labelled Polymer Agilent Cat#K4003 NP40 lysis buffer Thermo Fisher Scientific Cat# J60766.AP Bradford Assay Kit Thermo Fisher Scientific Cat#1863028 Brij35 Thermo Fisher Scientific Cat#85117 HaltTM protease inhibitor Thermo Fisher Scientific Cat#78430 Protein-Free Blocking Buffer Thermo Fisher Scientific Cat#37584 Streptavidin-AP SouthernBiotech Cat#7105-04 (Continued on next page) Immunity 59, 1–11.e1–e7, July 14, 2026 e2

    Glycoproteomics:

    Article Title: Methods and compositions for treating staphylococcal infections
    Article Snippet: .. To measure glycan residues in test antibodies, recombinant biotinylated lectins (Aleuria aurantia lectin, AAL; Lens culinaris agglutinin, LCA; Erythrina cristagalli lectin, ECL; Sambucus nigra lectin, SNA) were obtained from Vector Laboratories. ..

    Recombinant:

    Article Title: Methods and compositions for treating staphylococcal infections
    Article Snippet: .. To measure glycan residues in test antibodies, recombinant biotinylated lectins (Aleuria aurantia lectin, AAL; Lens culinaris agglutinin, LCA; Erythrina cristagalli lectin, ECL; Sambucus nigra lectin, SNA) were obtained from Vector Laboratories. ..



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